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| Test Name: | QuantiFERON TB Gold (In Tube) |
|---|---|
| Test Code: | 182873 |
| Other Names: | N/A |
| Test Includes: | QuantiFERON Incubation |
| Specimen Requirements: | Whole blood, 1 mL x three tubes (see Container) |
| Container: | The QuantiFERON(R) collection kit contains instructions for the draw of three special QuantiFERON(R) collection tubes (one each): (1) gray-top (with white ring), uncoated (nil); (2) red-top (with white ring), TB antigen-coated; (3) purple-top (with white ring), mitogen-coated. A high altitude kit is also available for locations between 3350 and 6150 feet. The tubes are differentiated by a cap with a yellow ring. |
| Collection Instructions: | Refer to collection instructions included with draw kit. Special specimen collection kit contains three gel-barrier tubes: gray-top/white ring (nil), red-top/white ring (TB antigens), and purple-top/white ring (mitogen). All three tubes are required for a single test result. Each tube is designed to draw only 1 mL and fill time may be longer than other blood collection tubes. Because of the limited vacuum in these tubes, use a needle and holder (NOT a butterfly) to collect QuantiFERON(R) specimens. If a butterfly is required, first collect other required tubes or use another Vacutainer(R) tube to purge the butterfly line of air and then proceed with drawing the "In QuantiFERON(R) tubes". Fill tubes to the black fill line on the tube. If tubes are underfilled or overfilled (see kit insert), immediately redraw a replacement tube. Following proper fill, label the tubes appropriately and shake tubes 10 times just firmly enough to ensure the entire surface of the tube is coated with blood cells to solubilize the antigen on the tube walls. After shaking, the volume may fall below the fill line. Do NOT centrifuge or refrigerate specimens. Return each of the 3 properly filled, labeled and shaken tubes to the box labeled "QFT(R) kit". Seal the top by removing tape from adhesive. Tubes should be incubated upright at 16-24 hours at 37 degrees C +/- 1 degree C in the box/kit within 16 hours of collection. To preserve cellular viability, specimens should be collected and sent same day, at room temperature, so as to arrive at the lab as soon as possible and within 14 hours of draw. Please indicate date and time of venipuncture on the tubes and on the test request form. If the specimen has been incubated for 16-24 hours at 37 degrees C +/- 1 degree C, please mark the top of the specimen box where indicated. |
| Stability: | Maintain specimen at room temperature (17 to 27 degrees C). Do NOT centrifuge, refrigerate, or ship tubes on ice. |
| Rejection Criteria / Special Instructions: | QuantiFERON is TIME-SENSITIVE and specimens must be received in the laboratory within 14 hours of collection. Specimen collection times will vary depending on logistics from the blood draw location to a LabCorp lab. This test requires a special collection |
| Methodology: | Mycobacterium tuberculosis antigen-stimulated interferon- gamma production with detection by enzyme-linked immunosorbent assay (ELISA). Test includes PHA (positive) and nil (negative) controls. This is also known as an interferon-gamma release assay (IGRA |