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Test Name: PT
Test Code: 1810
Other Names: Prothrombin Time Assay, PT
Test Includes: Prothrombin time
Specimen Requirements: Plasma
Container: Blue-top (sodium citrate) tube,90% of full draw
Collection Instructions: N/A
Stability: Specimens are stable at room temperature for 24 hours. If testing cannot be completed within 24 hours, specimens should be centrifuged for at least 10 minutes at 1500xg. Plasma should then be transferred to a transfer tube and frozen. Sample is stable when frozen for up to 2 weeks
Rejection Criteria / Special Instructions: Moderate to Gross hemolysis; clotted specimen; frozen specimen thawed in transit; tubes <90% full; improper labeling; specimen collected in tube other than 3.2% citrate
Methodology: The coagulation cascade is activated by incubating plasma with the optimal amount of thromboplastin and calcium; the clotting time is then measured.