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| Test Name: | PT |
|---|---|
| Test Code: | 1810 |
| Other Names: | Prothrombin Time Assay, PT |
| Test Includes: | Prothrombin time |
| Specimen Requirements: | Plasma |
| Container: | Blue-top (sodium citrate) tube,90% of full draw |
| Collection Instructions: | N/A |
| Stability: | Specimens are stable at room temperature for 24 hours. If testing cannot be completed within 24 hours, specimens should be centrifuged for at least 10 minutes at 1500xg. Plasma should then be transferred to a transfer tube and frozen. Sample is stable when frozen for up to 2 weeks |
| Rejection Criteria / Special Instructions: | Moderate to Gross hemolysis; clotted specimen; frozen specimen thawed in transit; tubes <90% full; improper labeling; specimen collected in tube other than 3.2% citrate |
| Methodology: | The coagulation cascade is activated by incubating plasma with the optimal amount of thromboplastin and calcium; the clotting time is then measured. |