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| Test Name: | Lupus Anticoagulant Reflex |
|---|---|
| Test Code: | 117892 |
| Other Names: | N/A |
| Test Includes: | PTT-LA |
| Specimen Requirements: | Plasma, FROZEN, 6 mL |
| Container: | Blue-top (sodium citrate) tube |
| Collection Instructions: | Citrated plasma samples should be collected by double centrifugation. Blood should be collected in a blue-top tube containing 3.2% buffered sodium citrate.[1] Evacuated collection tubes must be filled to completion to ensure a proper blood to anticoagulant ratio.[2,3] The sample should be mixed immediately by gentle inversion at least 6 times to ensure adequate mixing of the anti- coagulant with the blood. A discard tube is not required prior to collection of coagulation samples.[4,5] When noncitrate tubes are collected for other tests, collect sterile and nonadditive (red-top) tubes prior to citrate (blue-top) tubes. Any tube containing an alternate anticoagulant should be collected after the blue-top tubes. Gel-barrier tubes and serum tubes with clot initiators should also be collected after the citrate tubes. Centrifuge for 10 minutes and (using a plastic transfer pipette) carefully remove two thirds of the plasma without disturbing the cells. Deliver to a plastic transfer tube, cap, and recentrifuge for 10 minutes. Use a second plastic pipette to remove the plasma, staying clear of the platelets at the bottom of the tube. Transfer the plasma into a LabCorp PP transpak frozen purple tube with screw cap (LabCorp ID 49482). The specimen should be FROZEN immediately and maintained frozen until tested. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested. |
| Stability: | FREEZE |
| Rejection Criteria / Special Instructions: | N/A |
| Methodology: | PTT-LA (lupus-sensitive aPTT) and DRVVT screen; mixing study if screening tests are prolonged; confirmation if the mixing studies do not correct. |